MAUI Microarray Data

PRODUCTS » MAUI Product Suite » MAUI Mixer Hybridization Chambers » MAUI Microarray Data

Click to jump to a data section on this page

Increased Sensitivity using Significantly Less FFPE Sample

MAUI Hybridization System vs. Coverslip Using Invitrogen NCode miRNA Microarrays

MAUI Hybridization System vs. Coverslip Using Exiqon miRCURY LNA microRNA Arrays

MAUI Hybridization System vs. Coverslip Using Phalanx Biotech OneArray Microarrays

Increased Sensitivity & Precision Due to Active Mixing

Increased Sensitivity using Significantly Less FFPE Sample

Data Courtesy of: Myriad Genetics, Inc.

Experimental Details:
Agilent 244K CGH Array
FFPE DNA Input =
MAUI (1μg & 0.5 μg),
Competitors (2 μg & 1 μg)
Temperature = 55°C
Time = 40 hours
MAUI Mixer LC

	Competitor A
	Competitor B
	MAUI Hybridization System

MAUI Hybridization System vs. Coverslip Using Invitrogen NCode miRNA Microarrays

Data Courtesy of: Invitrogen Corporation

Experimental Details:
Invitrogen NCode Multispecies miRNA Array

Temperature = 52°C
Time = 16 hours
MAUI Mixer SC

Coverslip Hybridization	MAUI Hybridization System



The MAUI Hybridization System provides greatly enhanced sensitivity (up to 10 fold) and excellent inter-array reproducibility. Additionally, the intensities of positive features are greater than 10X median background intensity in both channels.

MAUI Hybridization System vs. Coverslip Using Exiqon miRCURY LNA microRNA Arrays

Data Courtesy of: Exiqon

Experimental Details:
Exiqon miRCURY LNA microRNA Arrays

Temperature = 56°C
Time = 16 hours
MAUI Mixer SL

Coverslip Hybridization	MAUI Hybridization System


Coverslip- Enlarged	MAUI Hybridization System- Enlarged


(A)	(B)


(C) Observed Differential Expression (MAUI vs. Coverslip)

(D) Spike-In Variation (MAUI vs. Coverslip)

The MAUI Hybridization System yields higher signal intensity & SNR compared to coverslip hybridization (Figures A & B) and higher differential expression as seen by the log-ratio vs. log-ratio plot comparing the 2 methods (Figure C). The MAUI Hybridization System also provides for less variation between spot replicates (Figure D)

MAUI Hybridization System vs. Coverslip Using Phalanx Biotech OneArray Microarrays

Data Courtesy of: Phalanx Biotech

Experimental Details:
Phalanx Biotech Mouse OneArrays

Temperature = 42°C
Time = 4 hours
MAUI Mixer SC

Coverslip Hybridization	MAUI Hybridization System



The MAUI Hybridization System yields significantly higher signal intensity compared to coverslip hybridization as seen by the Cy3/Cy5 intensity average and Log2 plot.

Increased Sensitivity & Precision Due to Active Mixing

Data Courtesy of: Patrick O. Brown Laboratory, Stanford University School of Medicine

Experimental Details:
Custom spotted 70-mer Oligo Yeast Expression Array

Temperature = 65°C
Time = 16 hours
MAUI Mixer SC

No Mixing	MAUI Hybridization System- Active Mixing


No Mixing R²= 0.850	Active Mixing R²= 0.940